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prostate carcinoma cell line du 145  (ATCC)


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    ATCC prostate carcinoma cell line du 145
    Prostate Carcinoma Cell Line Du 145, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prostate carcinoma cell line du 145/product/ATCC
    Average 99 stars, based on 9843 article reviews
    prostate carcinoma cell line du 145 - by Bioz Stars, 2026-03
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    Characterisation of prostate cancer-derived extracellular vesicles. EVs post differential ultracentrifugation were characterised using nanoparticle tracking analysis (NTA), immunoblotting analysis, and scanning electron microscopy (SEM). a ). Particle concentration and size of which three replicates of each sample were analysed by NTA independently. Data analysed by one-way ANOVA test and presented as mean bars (n = 3) ± SEM, the significant p-value is reported; * indicates p < 0.05, ** indicates p < 0.01. n.s. = not significant. b ) Representative graphs of prostate cancer EVs distribution from NTA software. c )Immunoblotting analysis of EVs from <t>PC-3,</t> LNCaP and DU 145 cells and cell lysates. Detection of EVs associated positive markers, syntenin, CD63, and negative marker calnexin. SEM images of d ) PC-3 EVs, e ) LNCaP EVs and f) DU 145 EVs with a range of 90 nm to 130 nm (magnification 62000x).
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    Characterisation of prostate cancer-derived extracellular vesicles. EVs post differential ultracentrifugation were characterised using nanoparticle tracking analysis (NTA), immunoblotting analysis, and scanning electron microscopy (SEM). a ). Particle concentration and size of which three replicates of each sample were analysed by NTA independently. Data analysed by one-way ANOVA test and presented as mean bars (n = 3) ± SEM, the significant p-value is reported; * indicates p < 0.05, ** indicates p < 0.01. n.s. = not significant. b ) Representative graphs of prostate cancer EVs distribution from NTA software. c )Immunoblotting analysis of EVs from PC-3, LNCaP and DU 145 cells and cell lysates. Detection of EVs associated positive markers, syntenin, CD63, and negative marker calnexin. SEM images of d ) PC-3 EVs, e ) LNCaP EVs and f) DU 145 EVs with a range of 90 nm to 130 nm (magnification 62000x).

    Journal: Scientific Reports

    Article Title: Immune profiling identifies the contribution of extracellular vesicles to immune modulation and progression in prostate cancer

    doi: 10.1038/s41598-025-31838-w

    Figure Lengend Snippet: Characterisation of prostate cancer-derived extracellular vesicles. EVs post differential ultracentrifugation were characterised using nanoparticle tracking analysis (NTA), immunoblotting analysis, and scanning electron microscopy (SEM). a ). Particle concentration and size of which three replicates of each sample were analysed by NTA independently. Data analysed by one-way ANOVA test and presented as mean bars (n = 3) ± SEM, the significant p-value is reported; * indicates p < 0.05, ** indicates p < 0.01. n.s. = not significant. b ) Representative graphs of prostate cancer EVs distribution from NTA software. c )Immunoblotting analysis of EVs from PC-3, LNCaP and DU 145 cells and cell lysates. Detection of EVs associated positive markers, syntenin, CD63, and negative marker calnexin. SEM images of d ) PC-3 EVs, e ) LNCaP EVs and f) DU 145 EVs with a range of 90 nm to 130 nm (magnification 62000x).

    Article Snippet: The androgen-dependent LNCaP metastatic prostate carcinoma cell line (CRL-1740), androgen-independent prostate carcinoma PC-3 cell line (CRL-1435) and androgen-independent prostate carcinoma DU 145 cell line (HTB-81) were purchased from the American Type Culture Collection (ATCC).

    Techniques: Derivative Assay, Western Blot, Electron Microscopy, Concentration Assay, Software, Marker

    Effect of PC-3 and LNCaP EVs on monocyte populations. PBMCs were treated with PC-3 EVs, LNCaP EVs, DU 145 EVs and t-NEPC LNCaP EVs for 24 h. a ) Comparison of % CD14 + CD16 + rare monocytes from 10 healthy volunteers treated with EVs derived from PC-3, LNCaP and DU 145. b ) Comparison of % CD14 + CD16 + rare monocytes treated with EVs derived from PC-3, LNCaP and DU 145 in respect to the untreated samples (CTRL). c ) Comparison of % CD14 + CD16 + rare monocytes treated with EVs derived from PC-3 compared to t-NEPC LNCaP treated cells. Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; **** p < 0.0001; *** indicates p < 0.001; n.s. = not significant.

    Journal: Scientific Reports

    Article Title: Immune profiling identifies the contribution of extracellular vesicles to immune modulation and progression in prostate cancer

    doi: 10.1038/s41598-025-31838-w

    Figure Lengend Snippet: Effect of PC-3 and LNCaP EVs on monocyte populations. PBMCs were treated with PC-3 EVs, LNCaP EVs, DU 145 EVs and t-NEPC LNCaP EVs for 24 h. a ) Comparison of % CD14 + CD16 + rare monocytes from 10 healthy volunteers treated with EVs derived from PC-3, LNCaP and DU 145. b ) Comparison of % CD14 + CD16 + rare monocytes treated with EVs derived from PC-3, LNCaP and DU 145 in respect to the untreated samples (CTRL). c ) Comparison of % CD14 + CD16 + rare monocytes treated with EVs derived from PC-3 compared to t-NEPC LNCaP treated cells. Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; **** p < 0.0001; *** indicates p < 0.001; n.s. = not significant.

    Article Snippet: The androgen-dependent LNCaP metastatic prostate carcinoma cell line (CRL-1740), androgen-independent prostate carcinoma PC-3 cell line (CRL-1435) and androgen-independent prostate carcinoma DU 145 cell line (HTB-81) were purchased from the American Type Culture Collection (ATCC).

    Techniques: Comparison, Derivative Assay

    PC-3-EVs effects on innate lymphoid and NK cells compared to LNCaP-EVs. Effect of prostate cancer EVs on innate immune cells. a ) The percentage of CD4 + HLA-DR + after treatment with EVs derived from PC-3 and DU 145 compared to LNCaP treatment. b ) The percentage of CD8 + HLA-DR + treated with EVs derived from PC-3 and DU 145 in comparison to LNCaP treatment. c ) Comparison of the ratio of CD4 + HLA-DR + regarding the CD4 + . d ) Comparison of ratio of CD8 + HLA-DR + in regard to the CD8 + . Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; * indicates p < 0.05; n.s. = not significant.

    Journal: Scientific Reports

    Article Title: Immune profiling identifies the contribution of extracellular vesicles to immune modulation and progression in prostate cancer

    doi: 10.1038/s41598-025-31838-w

    Figure Lengend Snippet: PC-3-EVs effects on innate lymphoid and NK cells compared to LNCaP-EVs. Effect of prostate cancer EVs on innate immune cells. a ) The percentage of CD4 + HLA-DR + after treatment with EVs derived from PC-3 and DU 145 compared to LNCaP treatment. b ) The percentage of CD8 + HLA-DR + treated with EVs derived from PC-3 and DU 145 in comparison to LNCaP treatment. c ) Comparison of the ratio of CD4 + HLA-DR + regarding the CD4 + . d ) Comparison of ratio of CD8 + HLA-DR + in regard to the CD8 + . Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; * indicates p < 0.05; n.s. = not significant.

    Article Snippet: The androgen-dependent LNCaP metastatic prostate carcinoma cell line (CRL-1740), androgen-independent prostate carcinoma PC-3 cell line (CRL-1435) and androgen-independent prostate carcinoma DU 145 cell line (HTB-81) were purchased from the American Type Culture Collection (ATCC).

    Techniques: Derivative Assay, Comparison

    Release of cytokines by immune cells after treatment with prostate cancer EVs. Seven pro- and anti-inflammatory cytokines were evaluated from the conditioned medium of PBMCs from 10 healthy donors and samples treated with PC-3-EVs, LNCaP-EVs and DU 145 EVs for 24h. a ) Level of IL-6 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. b ) Level of MIP-1α between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. c ) Level of IL-7 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. d ) Level of IFN-α2 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. e ) Level of IL-10 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. f ) Level of MCP-1 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. g ) Level of G-CSF between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; **** indicates p < 0.0001; ***indicates p < 0.001; ** indicates p < 0.01; *indicates p < 0.05.

    Journal: Scientific Reports

    Article Title: Immune profiling identifies the contribution of extracellular vesicles to immune modulation and progression in prostate cancer

    doi: 10.1038/s41598-025-31838-w

    Figure Lengend Snippet: Release of cytokines by immune cells after treatment with prostate cancer EVs. Seven pro- and anti-inflammatory cytokines were evaluated from the conditioned medium of PBMCs from 10 healthy donors and samples treated with PC-3-EVs, LNCaP-EVs and DU 145 EVs for 24h. a ) Level of IL-6 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. b ) Level of MIP-1α between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. c ) Level of IL-7 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. d ) Level of IFN-α2 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. e ) Level of IL-10 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. f ) Level of MCP-1 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. g ) Level of G-CSF between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; **** indicates p < 0.0001; ***indicates p < 0.001; ** indicates p < 0.01; *indicates p < 0.05.

    Article Snippet: The androgen-dependent LNCaP metastatic prostate carcinoma cell line (CRL-1740), androgen-independent prostate carcinoma PC-3 cell line (CRL-1435) and androgen-independent prostate carcinoma DU 145 cell line (HTB-81) were purchased from the American Type Culture Collection (ATCC).

    Techniques:

    Characterisation of prostate cancer-derived extracellular vesicles. EVs post differential ultracentrifugation were characterised using nanoparticle tracking analysis (NTA), immunoblotting analysis, and scanning electron microscopy (SEM). a ). Particle concentration and size of which three replicates of each sample were analysed by NTA independently. Data analysed by one-way ANOVA test and presented as mean bars (n = 3) ± SEM, the significant p-value is reported; * indicates p < 0.05, ** indicates p < 0.01. n.s. = not significant. b ) Representative graphs of prostate cancer EVs distribution from NTA software. c )Immunoblotting analysis of EVs from PC-3, LNCaP and DU 145 cells and cell lysates. Detection of EVs associated positive markers, syntenin, CD63, and negative marker calnexin. SEM images of d ) PC-3 EVs, e ) LNCaP EVs and f) DU 145 EVs with a range of 90 nm to 130 nm (magnification 62000x).

    Journal: Scientific Reports

    Article Title: Immune profiling identifies the contribution of extracellular vesicles to immune modulation and progression in prostate cancer

    doi: 10.1038/s41598-025-31838-w

    Figure Lengend Snippet: Characterisation of prostate cancer-derived extracellular vesicles. EVs post differential ultracentrifugation were characterised using nanoparticle tracking analysis (NTA), immunoblotting analysis, and scanning electron microscopy (SEM). a ). Particle concentration and size of which three replicates of each sample were analysed by NTA independently. Data analysed by one-way ANOVA test and presented as mean bars (n = 3) ± SEM, the significant p-value is reported; * indicates p < 0.05, ** indicates p < 0.01. n.s. = not significant. b ) Representative graphs of prostate cancer EVs distribution from NTA software. c )Immunoblotting analysis of EVs from PC-3, LNCaP and DU 145 cells and cell lysates. Detection of EVs associated positive markers, syntenin, CD63, and negative marker calnexin. SEM images of d ) PC-3 EVs, e ) LNCaP EVs and f) DU 145 EVs with a range of 90 nm to 130 nm (magnification 62000x).

    Article Snippet: The androgen-dependent LNCaP metastatic prostate carcinoma cell line (CRL-1740), androgen-independent prostate carcinoma PC-3 cell line (CRL-1435) and androgen-independent prostate carcinoma DU 145 cell line (HTB-81) were purchased from the American Type Culture Collection (ATCC).

    Techniques: Derivative Assay, Western Blot, Electron Microscopy, Concentration Assay, Software, Marker

    Effect of PC-3 and LNCaP EVs on monocyte populations. PBMCs were treated with PC-3 EVs, LNCaP EVs, DU 145 EVs and t-NEPC LNCaP EVs for 24 h. a ) Comparison of % CD14 + CD16 + rare monocytes from 10 healthy volunteers treated with EVs derived from PC-3, LNCaP and DU 145. b ) Comparison of % CD14 + CD16 + rare monocytes treated with EVs derived from PC-3, LNCaP and DU 145 in respect to the untreated samples (CTRL). c ) Comparison of % CD14 + CD16 + rare monocytes treated with EVs derived from PC-3 compared to t-NEPC LNCaP treated cells. Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; **** p < 0.0001; *** indicates p < 0.001; n.s. = not significant.

    Journal: Scientific Reports

    Article Title: Immune profiling identifies the contribution of extracellular vesicles to immune modulation and progression in prostate cancer

    doi: 10.1038/s41598-025-31838-w

    Figure Lengend Snippet: Effect of PC-3 and LNCaP EVs on monocyte populations. PBMCs were treated with PC-3 EVs, LNCaP EVs, DU 145 EVs and t-NEPC LNCaP EVs for 24 h. a ) Comparison of % CD14 + CD16 + rare monocytes from 10 healthy volunteers treated with EVs derived from PC-3, LNCaP and DU 145. b ) Comparison of % CD14 + CD16 + rare monocytes treated with EVs derived from PC-3, LNCaP and DU 145 in respect to the untreated samples (CTRL). c ) Comparison of % CD14 + CD16 + rare monocytes treated with EVs derived from PC-3 compared to t-NEPC LNCaP treated cells. Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; **** p < 0.0001; *** indicates p < 0.001; n.s. = not significant.

    Article Snippet: The androgen-dependent LNCaP metastatic prostate carcinoma cell line (CRL-1740), androgen-independent prostate carcinoma PC-3 cell line (CRL-1435) and androgen-independent prostate carcinoma DU 145 cell line (HTB-81) were purchased from the American Type Culture Collection (ATCC).

    Techniques: Comparison, Derivative Assay

    PC-3-EVs effects on innate lymphoid and NK cells compared to LNCaP-EVs. Effect of prostate cancer EVs on innate immune cells. a ) The percentage of CD4 + HLA-DR + after treatment with EVs derived from PC-3 and DU 145 compared to LNCaP treatment. b ) The percentage of CD8 + HLA-DR + treated with EVs derived from PC-3 and DU 145 in comparison to LNCaP treatment. c ) Comparison of the ratio of CD4 + HLA-DR + regarding the CD4 + . d ) Comparison of ratio of CD8 + HLA-DR + in regard to the CD8 + . Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; * indicates p < 0.05; n.s. = not significant.

    Journal: Scientific Reports

    Article Title: Immune profiling identifies the contribution of extracellular vesicles to immune modulation and progression in prostate cancer

    doi: 10.1038/s41598-025-31838-w

    Figure Lengend Snippet: PC-3-EVs effects on innate lymphoid and NK cells compared to LNCaP-EVs. Effect of prostate cancer EVs on innate immune cells. a ) The percentage of CD4 + HLA-DR + after treatment with EVs derived from PC-3 and DU 145 compared to LNCaP treatment. b ) The percentage of CD8 + HLA-DR + treated with EVs derived from PC-3 and DU 145 in comparison to LNCaP treatment. c ) Comparison of the ratio of CD4 + HLA-DR + regarding the CD4 + . d ) Comparison of ratio of CD8 + HLA-DR + in regard to the CD8 + . Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; * indicates p < 0.05; n.s. = not significant.

    Article Snippet: The androgen-dependent LNCaP metastatic prostate carcinoma cell line (CRL-1740), androgen-independent prostate carcinoma PC-3 cell line (CRL-1435) and androgen-independent prostate carcinoma DU 145 cell line (HTB-81) were purchased from the American Type Culture Collection (ATCC).

    Techniques: Derivative Assay, Comparison

    T-NEPC LNCaP EVs effects on innate lymphoid and NK cells. Effect of t-NEPC LNCaP EVs on innate immune cells. a ) The percentage of CD4 + HLA-DR + after treatment with EVs derived from PC-3 compared to t-NEPC LNCaP treatment. b ) The percentage of CD8 + HLA-DR + treated with EVs derived from PC-3 compared to t-NEPC LNCaP treatment. c ) Comparison of the ratio of CD4 + HLA-DR + regarding the CD4 + . d ) Comparison of ratio of CD8 + HLA-DR + in regard to the CD8 + . Data analysed by parametric t-test and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; * indicates p < 0.05; n.s. = not significant.

    Journal: Scientific Reports

    Article Title: Immune profiling identifies the contribution of extracellular vesicles to immune modulation and progression in prostate cancer

    doi: 10.1038/s41598-025-31838-w

    Figure Lengend Snippet: T-NEPC LNCaP EVs effects on innate lymphoid and NK cells. Effect of t-NEPC LNCaP EVs on innate immune cells. a ) The percentage of CD4 + HLA-DR + after treatment with EVs derived from PC-3 compared to t-NEPC LNCaP treatment. b ) The percentage of CD8 + HLA-DR + treated with EVs derived from PC-3 compared to t-NEPC LNCaP treatment. c ) Comparison of the ratio of CD4 + HLA-DR + regarding the CD4 + . d ) Comparison of ratio of CD8 + HLA-DR + in regard to the CD8 + . Data analysed by parametric t-test and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; * indicates p < 0.05; n.s. = not significant.

    Article Snippet: The androgen-dependent LNCaP metastatic prostate carcinoma cell line (CRL-1740), androgen-independent prostate carcinoma PC-3 cell line (CRL-1435) and androgen-independent prostate carcinoma DU 145 cell line (HTB-81) were purchased from the American Type Culture Collection (ATCC).

    Techniques: Derivative Assay, Comparison

    Release of cytokines by immune cells after treatment with prostate cancer EVs. Seven pro- and anti-inflammatory cytokines were evaluated from the conditioned medium of PBMCs from 10 healthy donors and samples treated with PC-3-EVs, LNCaP-EVs and DU 145 EVs for 24h. a ) Level of IL-6 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. b ) Level of MIP-1α between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. c ) Level of IL-7 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. d ) Level of IFN-α2 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. e ) Level of IL-10 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. f ) Level of MCP-1 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. g ) Level of G-CSF between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; **** indicates p < 0.0001; ***indicates p < 0.001; ** indicates p < 0.01; *indicates p < 0.05.

    Journal: Scientific Reports

    Article Title: Immune profiling identifies the contribution of extracellular vesicles to immune modulation and progression in prostate cancer

    doi: 10.1038/s41598-025-31838-w

    Figure Lengend Snippet: Release of cytokines by immune cells after treatment with prostate cancer EVs. Seven pro- and anti-inflammatory cytokines were evaluated from the conditioned medium of PBMCs from 10 healthy donors and samples treated with PC-3-EVs, LNCaP-EVs and DU 145 EVs for 24h. a ) Level of IL-6 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. b ) Level of MIP-1α between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. c ) Level of IL-7 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. d ) Level of IFN-α2 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. e ) Level of IL-10 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. f ) Level of MCP-1 between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. g ) Level of G-CSF between PBMCs treated with PC-3 EVs and DU 145 EVs compared to LNCaP EVs. Data analysed by one-way ANOVA and presented as mean bars (n = 10) ± SEM, the significant p -value is reported; **** indicates p < 0.0001; ***indicates p < 0.001; ** indicates p < 0.01; *indicates p < 0.05.

    Article Snippet: The androgen-dependent LNCaP metastatic prostate carcinoma cell line (CRL-1740), androgen-independent prostate carcinoma PC-3 cell line (CRL-1435) and androgen-independent prostate carcinoma DU 145 cell line (HTB-81) were purchased from the American Type Culture Collection (ATCC).

    Techniques: